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عمومی::
تشدید پلاسمون سطحی، رزونانس پلاسمون سطحی
In some embodiments, the method for determining the KD of an antibody is by using surface plasmon resonance, for example, by using a biosensor system such as a BIACORE(r) system.
Examples of such assays include, although not limited to: enzyme-linked immunosorbent assay (ELISA), enzyme immunoassay (EIA), surface plasmon resonance (SPR), fluorescence resonance energy transfer (FRET), and luminescence resonance energy transfer (LRET).
[00252] Competitive binding studies can be done as is known in the art, generally using SPR/Biacore(r) or Octet(r) binding assays, as well as ELISA and cell based assays.
Example 4: Analysis of binding affinity by SPR
[00323] Some of the humanized antibodies having combinations of VL and VH shown in Table 3A were subjected to binding affinity analysis by surface plasmon resonance (SPR) using chimeric antibody Ta1505 as a reference antibody.،They commonly possess facile conjugation sites to diverse biomolecule ligands and antibodies, remarkable surface plasmon resonance (SPR) properties, superior optical, and photothermal conversion properties [61-63].
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